Daya Antibakteri Ekstrak Daun Api-Api (Avicennia alba) terhadap Vibrio harveyi Penyebab Vibriosis secara Invitro In Vitro Antibacteria Activity of Api-Api (Avicennia alba) Leave Extract Against Vibrio harveyi Causes Vibriosis

摘要

AbstrakBudidaya udang dihadapkan pada berbagai kendala penyakit yang timbul dan  dapat menyebabkan kematian masal pada larva udang windu, salah satunya  vibriosis yang   disebabkan oleh bakteri Vibrio harveyi.   Zat antibakteri yaitu : flavonoid, saponin dan tanin terbukti dapat membunuh beberapa bakteri patogen.  Daun api – api Avicennia alba mengandung zat anti bakteri sehingga berpotensi digunakan untuk membunuh bakteri Vibrio harveyi. Penelitian ini bertujuan untuk mengetahui konsentrasi optimum ekstrak daun api-api (Avicennia alba) dalam menghambat dan membunuh pertumbuhan bakteri Vibrio harveyi. Penelitian ini  mengunakan metode eksperimental dengan  perlakuan konsentrasi ekstrak daun api-api (Avicennia alba) sebesar  100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%.  Parameter yang diamati  dalam penelitian ini  adalah Minimum Inhibitory Concentration (MIC) dan pertumbuhan koloni  pada uji Minimum Bacteria Concentration (MBC).  Hasil penelitian menunjukan bahwa pada  konsentrasi 60% ekstrak daun Avicennia alba dapat menghambat pertumbuhan  V.harveyi. Sedangkan pada konsentrasi 90% ekstrak daun Avicennia alba menyebabkan  bakteri V.harveyi terbunuh.   AbstractShrimp culture has various obstacles such as the emergence of diseases that can cause deaths in tiger shrimp larvae such as vibriosis caused by Vibrio harveyi. Some antibacterial substances such as flavonoid, saponin, and tannin inhibit and kill several pathogenic bacteria. Avicennia alba leaves contain anti-bacterial substances that have the potential to be used to kill Vibrio harveyi bacteria. This study aimed to determine the minimum concentration of Avicennia alba leaves extract for inhibiting and kill the growth of Vibrio harveyi. This study used an experimental method, using  Avicennia alba leaves extract concentration treatment of 100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%. The parameters observed in this study were the Minimum Inhibitory Concentration (MIC) and bacteria colony growth in Minimum Bacteria Concentration (MBC) test. The results showed that at a concentration of 60% Avicennia alba leaves extract could inhibit the growth of V.harveyi. While at a concentration of 90% Avicennia alba leaves extract, Vibrio harveyi bacteria was killed.