The determination of regional blood flow utilizing fluorescent microspheres (FMs) is an established method for numerous organs. Recent progress, in particular the automation of sample processing, has further improved this method. However, the FM method (reference sample technique), which allows repetitive measurement of regional organ blood flow, has so far not been used for the determination of blood flow in bone. The aim of the present study was to establish FM for the quantification of regional bone blood flow (RBBF). Female, anesthetized New Zealand rabbits (n = 6) received left ventricular injections of different amounts of FM at six subsequent time points. In order to examine the precision of RBBF determination, two different FM species were injected simultaneously at the sixth injection. At the end of the experiments the femoral and tibial condyles of each hind limb were removed and the fluorescence intensity in the tissue samples was measured by an automated procedure. In an in vitro study we have shown that acid digestion of the crystalline matrix has no effect on the fluorescence characteristics of FM. The determination of the number of spheres per tissue sample revealed that depending on the tissue sample size up to 3 x 10(6) spheres/injection were necessary to obtain about 400 microspheres in the individual bone samples. RBBF values of the tibial and femoral condyles did not differ at various injection intervals. The tibial blood flow values varied between 6.6 +/- 1.1 and 8.5 +/- 1.4 ml/min/100 g and were significantly higher than those of the femur (4.3 +/- 1.1 to 6.0 +/- 1.8 ml/min/100 g). The bone blood flow values obtained by simultaneous injection of two FM species correlated significantly (r = 0.96, slope = 1.06, intercept = 0.05), the mean difference was 0.39 +/- 1.11 ml/min/100 g. Our data demonstrate that the measurement of RBBF by means of FM allows a valid determination of RBBF. Copyright (C) 2003 S. Karger AG, Basel.
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机译：利用荧光微球（FMs）确定区域血流量是许多器官的既定方法。最近的进展，特别是样品处理的自动化，进一步改进了该方法。但是，至今仍未使用FM方法（参考样本技术）来重复测量局部器官的血流，但该方法尚未用于确定骨骼中的血流。本研究的目的是建立用于定量局部骨血流（RBBF）的FM。雌性，麻醉的新西兰兔（n = 6）在随后的六个时间点接受左心室注射不同量的FM。为了检查RBBF测定的准确性，在第六次进样中同时注射了两种不同的FM物质。在实验结束时，去除每个后肢的股骨和胫骨con，并通过自动化程序测量组织样品中的荧光强度。在体外研究中，我们显示了酸解结晶基质对FM的荧光特性没有影响。确定每个组织样本的球数表明，根据组织样本的大小，最多需要3 x 10（6）个球/注射液才能在单个骨骼样本中获得约400个微球。胫骨和股骨dy的RBBF值在不同的注射间隔下没有差异。胫骨血流值在6.6 +/- 1.1和8.5 +/- 1.4 ml / min / 100 g之间变化，并且显着高于股骨的血流值（4.3 +/- 1.1到6.0 +/- 1.8 ml / min / 100 G）。通过同时注射两种FM物种获得的骨血流值显着相关（r = 0.96，斜率= 1.06，截距= 0.05），平均差为0.39 +/- 1.11 ml / min / 100 g。我们的数据表明，通过FM测量RBBF可以有效确定RBBF。版权所有（C）2003 S.Karger AG，巴塞尔。
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