BIODEGRADATION OF MONOCLHOROTRIAZINYL REACTIVE RED BY PSEUDOMONAS rudinensis and PSEUDOMONAS diminuta

摘要

Microorganism was isolated from polluted sludge in textile industiy wastewater discharge channel. Isolated microorganisms to be acclimated using Monochioromazynil compound and then pun fication process was canied out The result from purification of microorganisms is bacteria was named: Pseudomonas nidinensis and Pseudomonas diminuta. Biodegradation of Psedomonas rudinensis and Pseudornonas diminuta on red colour Monochiorotriazynil (Mkt) analysis using Gas Chromatography showed that both of bacteria where able to degrade red colour Mkta on aerobic condition. This condition was shown by degradation of Mkta concentration from initial concentration (.10 mg/I, 25 mg/I. 50 mgI! and 75 mg/I). Over 108 hours reaction time on the both bacteria Biodegradation result analysis using Gas Chromatography Mass Spectra (GC-MS) showed series of Mkta biodegradation evens by breaking reactive ring and connecting ring that was done by .Psedomonas rudinensis in to 4- Metoxyaniline. After the breakage of ring, the brekage of azo ring as a chromophore ring (colour carrier) into to compound, 2 Nahiumsulfonat 4-Metoxyaniline and 2 Amino 3-Natriumsulfonat AlfanaftoL This even was maxked by degradation of red colour. These two compounds broke into 4-Metoxyaniline and 2 Naphteleneamine Aniline. All of these biodegradation series used Mkta compound as both carbon and energy sources for bacteria’s growth