Evaluation and selection of optimal solvent and solvent combinations in thin-Iayer chromatography of flavonoids and of phenolic acids of Zizyphus jujuba MilI.

摘要

A series of simple mathematical techniques for the evaluation ofsolvents and solvent combinations in thin-layer chromatographyhave been investigated. The classification has been carried out usingthe numerical taxonomy techniques and information contentderived from Shannon's equation. The methods have been appliedto an RF data set of flavonoids and phenolic acids identified in themethanolic extract of Zizyphus jujuba MilI. The most suitable chromatographic systems for the separation and future isolation of flavonoids and phenolic acids from Zizyphus jujuba Mill. are : ethylacetate : formic acid : acetic acid : water, 100 : 11 : 11 : 27, andethyl acetate : formic acid : water, 8 : 1 : 1.