Xurography actuated valving for arbitrary timing of centrifugal flow control in parallelized multi-step bioassays

摘要

Here we introduce a new, instrument controlled valving scheme for the centrifugal platform whichudis based upon dissolvable film (DF) technology. Liquid, restrained at any point upon the disc, is preventedudfrom wetting a DF via a trapped gas pocket. From this pocket a pneumatic channel runs to audsealed vent located on the top surface of the disc. Controlled scouring of this seal by a robotic knifecutterudpermits venting of the trapped gas, and thus actuation of the valve. To demonstrate the potentialudof these valves, we present a disc developed towards a biplex liver assay panel.