The canine hookworm genome: analysis and classification of Ancylostoma caninum survey sequencesud

摘要

Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model udfor studying human infections and information from its genome coupled with functional genomics udand proteomics can accelerate progress towards hookworm control. As a step towards a full-scale udA. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined udthe genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a udgenome that we estimate by flow cytometry of isolated nuclei to be 347±1.2 Mb, substantially larger udthan other Rhabditina species. Gene finding identified 5,538 genes in the GSS assembly, for a total udof 9,113 non-redundant A. caninum genes when EST sequences are also considered. Functional udclassifications of many of the 70% of genes with homology to genes in other species are provided udbased on Gene Ontology and KEGG associations and secreted and membrane-bound proteins are udalso identified. udud