Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations

摘要

Gold nanoparticles decorated with full-length sialic acid terminatedcomplex bi-antennary N-glycans, synthesized with glycansisolated from egg yolk, were used as a sensor for the detectionof both recombinant hemagglutinin (HA) and whole influenzaA virus particles of the H1N1 subtype. Nanoparticle aggregationwas induced by interaction between the sialic acidtermini of the glycans attached to gold and the multivalentsialic acid binding sites of HA. Both dynamic light scattering (DLS) and UV/Vis spectroscopy demonstrated the efficiency of the sensor, which could detect viral HA at nanomolar concentrationsand revealed a linear relationship between the extentof nanoparticle aggregation and the concentration of HA. UV/Vis studies also showed that these nanoparticles can selectivelydetect an influenza A virus strain that preferentially binds sialicacid terminated glycans with a(2!6) linkages over a strainthat prefers glycans with terminal a(2!3)-linked sialic acids.