Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase

摘要

Background: Novozym 435, a commercial lipase from Candida antarctica, recombinant, expressed in Aspergillus niger,immobilized on macroporous acrylic resin, has been already described in the obtention of biodiesel. It is here evaluatedin the production of a new biofuel that integrates the glycerol as monoglyceride (MG) together with two fatty acidethyl esters (FAEE) molecules by the application of 1,3-selective lipases in the ethanolysis reaction of sunflower oil.Results: Response surface methodology (RSM) is employed to estimate the effects of main reaction. Optimumconditions for the viscosity, selectivity, and conversion were determined using a multifactorial design ofexperiments with three factors run by the software Stat Graphics version XV.I. The selected experimentalparameters were reaction temperature, oil/ethanol ratio and alkaline environment. On the basis of RSM analysis,the optimum conditions for synthesis were 1/6 oil/EtOH molar ratio, 30°C, and 12.5 μl of NaOH 10 N aqueoussolutions, higher stirring than 300 rpm, for 2 h and 0.5 g of biocatalyst.Conclusions: These obtained results have proven a very good efficiency of the biocatalyst in the studied selectiveprocess. Furthermore, it was allowed sixteen times the successive reuse of the biocatalyst with good performance