A rapid and high-throughput quantum dots bioassay for monitoring ofperfluorooctane sulfonate in environmental water samples

摘要

Currently HPLC/MS is the state of the art tool for environmental/drinking waterperfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomalproliferator-activated receptor-alpha (PPARα), which forms heterodimers withretinoid X receptors (RXRs) and binds to PPAR response elements. In thisbioassay free PFOS in water samples competes with immobilized PFOS in ELISAplates for a given amount of PPARα-RXRα. It can be determined indirectly byimmobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibodyand subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα-RXRα probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5ngL-1 with linear rangebetween 2.5ngL-1 and 75ngL-1. Detection results of environmental water sampleswere highly consistent between the bioassay an