Responses of air humidity and light quality on growth and stomata function of greenhouse grown Rosa*hybrida

摘要

Single node stem segment with one mature leaf of Rosa x hybrida, cv. Toril were grown inchambers. Plants were exposed to 100 μmol m-2 s-1 for 20 h day-1 followed by a 4 h dark periodand supplementary light was provided by LED (20% blue light and 80% red light), HPS andHPS+UV-B Lamp. Each light source was provided by moderate (60%) and high (90) RH indifferent chambers. The UV-B light was provided 1 hour twice a day, in the middle of the darkperiod and in the middle of light period. The UV-B treatment was given in combination withHPS lamp as supplementary light. Thus, the main aim of the study was to investigate if lightquality can improve the stomata function under high RH.The experiment was divided into two parts, first HPS and LED and seconds the effect of UV-B.In the first experiment, the stems were significantly longer under HPS compared to LED, undermoderate RH, LED suppressed pedicel length and induced shorter pedicels compared to HPS.The number of flowers and leaf area was not significantly affected by either RH or light quality.Comparatively, moderate RH had significantly more leaves per stem higher, dry weight of stemand higher dry weight of pedicle length and flower compared to high RH, but no significanteffect of light quality was found on these parameters. At high RH, gsw was still high duringdarkness for both HPS and LED and indicate lack of dark induced closure. However, LEDreduced the conductance under high RH during light and darkness indicating reduced stomatalaperture and a better closure capacity when grown with LED. Moderate RH had a lowerconductance under LED compared to HPS but both light qualities induced closure in darkness.Detached leaves from plants grown at high RH showed a rapid water loss compare to leavesgrown at moderate RH. Stomatal length and pore aperture were significantly higher in plantsgrown at high RH compared to moderate RH. Plants developed at high RH with LED showed adesiccation tolerance than plant grown under high RH and HPS. In second experiment, both highand moderate humidity showed a similar pattern with the conductance decreasing through thetime of one hour exposure of UV-B. The UV-B did not induce better closure rather the oppositewas found and plants developed at high RH and exposed to UV-B had a higher water loss in adesiccation test. Comparison of flavonoids and anthocynins on high and moderate humidity ofiiiUV-B treatment was found to be non-significant. Hence, the conclusion of this study is that lightquality can be used a tool to improve the stomata function under high RH. By using LED withmore blue light (20%) a better dark induced closure was found and a better desiccation tolerancewas found compared to the traditional HPS lamps. The use of UV-B did not improve the stomatafunction under high RH but rather induced an even higher water loss. The interactive effectbetween light quality and air humidity needs further investigation but light quality can be one ofthe useful tools since LEDs are believed to be an important light source in greenhouse in thefuture.