重组戊型肝炎病毒衣壳蛋白p239特异性吸附、侵入HepG2细胞并阻断野病毒对肝细胞的感染

摘要

By using Western blot and immunofluorescence assays,the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature.Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells,indicating that p239 and HEV competed the same targeting site on these cells.These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.