Utilization of Catharanthus Roseus Hairy Root and Cell Suspension Cultures in Plant Biotechnology.

摘要

Plant cell culture technology finds increasing use in agricultural crop improvement, germplasm storage and commercial micropropagation as well as in fundamental studies of plant biochemistry, physiology and genetics. The production of useful chemicals by plant cell and tissue cultures is also an area of intensive study. In the present study, a methodology for the establishment and cultivation of hairy root cultures of C. roseus both in shake flasks and a small fermenter was developed. The applicability of hairy root cultures for production of indole alkaloids was evaluated in comparison with cell suspension cultures. Furthermore, the effect of temperature on both culture types was studied, and the usefulness of plant cell culture technology in studying temperature adaption mechanisms of plants was evaluated.