Macromolecular Adduction by Trichloroacetonitrile in the Fischer 344 Rat Following Oral Gavage.

摘要

Male Fisher 344 rats were administered 1- or 2-((14)C)trichloroacetonitrile (TCAN) by oral gavage. DNA was isolated from the liver, kidneys and stomach and several protein fractions (globin, albumin and globulins) were isolated from blood. The stomach exhibited the highest level of DNA binding, followed in order by the liver and kidney. TCAN binding level was higher in DNA isolated from rats killed at 24 h than at 4 h after administration. Radioactivity associated with DNA was not incorporated into the nitrogen bases (i.e. via de novo synthesis) and a covalent binding index (micromol chemical bound/mol nucleotide phosphate per mmol/kg body wt. of chemical administered) of 30 - 120 was observed for various tissues.