Toxicity Studies of Trinitrofluorenone (CAS No. 129-79-3) Administered by TopicalApplication and Dosed Feed to F344/N Rats and B6C3F1 Mice

摘要

Trinitrofluorenone (TNF) is a major component of a toning formulation that at onetime was used widely in certain photocopy processes. Because the principal route of exposure of humans to TNF probably would be dermal, studies were conducted to compare chemical absorption, distribution, excretion, and tissue retention, as well as toxicity in 14-day studies, by oral and dermal routes of exposure. Further, 13-week toxicity studies were carried out with TNF incorporated into the feed of rats and mice of both sexes. In genetic toxicity evaluations, TNF was found to be mutagenic in Salmonella typhimurium, with and without metabolic activation. TNF caused a variety of lesions in oral feeding studies. The no-observed-adverse-effect-level (NOAEL) for microscopic changes other than pigment accumulation was 1000 ppm for rats. A NOAEL could not be determined for mice from the study. Limited dermal absorption likely would prevent significant systemic toxicity resulting from contact of TNF with the skin.