ENU Mutagenesis in the Mouse Electrophoretic Specific-Locus Test. 2. MutationalStudies of Mature Oocytes

摘要

Experiments were conducted using the biochemical specific-locus test to assessthe mutagenicity of N-ethyl-N-nitrosourea (ENU) in mature oocytes of mice. C57Bl/6J females were treated with 100 mg/kg ENU by intraperitoneal injection and mated to untreated DBA males for 1 week following treatment. 1447 progeny were screened for evidence of mutations affecting the electrophoretic mobility of 32 different proteins; two mutants were detected by electrophoretic analyses. These results provide evidence that ENU is a germ-cell mutagen in mouse mature oocytes, although the frequency of mutants is somewhat lower than that obtained from spermatogonia treated with the same dose. (Copyright (c) 1992 Elsevier Science Publishers B.V.)