PCR Primers Specific for Detection of a Rat Repetitive Sequence

摘要

Identification of a new oncogene is often accomplished by transfection of genomicDNA of interest into a host of a different species that can respond to the altered phenotype. We report a method for tracking and identifying potential genes of interest that are derived from rat DNA. We used polymerase chain reaction to amplify specifically the rat long-interspersed repetitive sequences in mouse cells that were transfected with rat DNA.