Stable RNA Sequences as a Tool for DNA Probes and Phylogenetic Studies

摘要

In the work, several different detection systems based on 16S rRNA sequences werestudied. The rRNA sequences were also used to infer phylogenetic relationships. The catalytic RNA from RNase P was studied as a potential alternative to rRNA sequences. Species-specific rDNA probes for Mycoplasma agalactiae, Mycoplasma bovis, Mycoplasma hyopneumoniae and Renibacterium salmoninarum were designed. The different probes were specific and could be used to identify their respective targets in clinical samples. It was observed that unexpected cross-hybridization of a probe to a non-target organism may depend on the type of mismatch. M. agalactiae is the closest relative to M. bovis, as was concluded from phylogenetic analysis. Group-specific rDNA probes for the detection of mycoplasma contaminated cell cultures was developed and tested on a large number of samples. (Copyright (c) Tryck: Sveriges lantbruksuniversitet, Uppsala 1993.)