Sea Urchin ('Strongylocentrotus purpuratus') Fertilization Test Method

摘要

The method measures the toxicity of effluents and receiving water to the gametesof a sea urchin, Strongylocentrotus purpuratus, during a 1 h sperm exposure and a subsequent 20 min exposure period following the addition of eggs for measuring the fertilizing capacity of the sperm. The purpose of the test is to determine the concentrations of a test substance that reduce egg fertilization by exposed sperm relative to that attained by sperm in control solutions.