Effects of Selected Neuroactive Chemicals on Calcium Transporting Systems in RatCerebellum and on Survival of Cerebellar Granule Cells

摘要

This investigation examined the relationship between alteration of Ca(2+)-transport systems and cytotoxicity in vitro for a number of neuroactive chemicals including environmental pollutants. (45)Ca(2+) uptake as a measure of Ca(2+) sequestration was determined in mitochondria and microsomes isolated from cerebella of adult male Long-Evans hooded rats by differential centrifugation. Ca(2+) extrusion, measured as Ca(2+)-ATPase activity, was determined in synaptosomes prepared by sucrose density gradient. Cytotoxicity (lactate dehydrogenase leakage) was assessed in primary cultures of cerebellar granule