Toxicokinetics and Structure-Activity Relationships of Nine Para-Substituted Phenols in Rat Embryos In vitro

摘要

The purpose of the study was to examine the toxicokinetics of embryo uptake following exposure to a variety of chemically related phenols in rat embryo culture. The uptake of nine radiolabeled para-substituted phenols by day 10 (9-13 somite stage) rat embryos in vitro was determined from 1 to 42 hrs after being placed in culture media containing various phenols. When hepatocytes were also present in the media the equilibrium concentration in the embryos was less than when hepatocytes were absent. Thus the metabolites produced by the hepatocytes appeared to have less affinity for the embryo than the parent phenol. Toxicodynamic information as given by the effective concentration of the phenol in the embryo to cause somite or tail teratological effects was best predicted by the measured unbound fraction. (Copyright (c) 1993 Wiley-Liss, Inc.)