Mutation Spectra of Glu-P-1 in Salmonella: Induction of Hotspot Frameshifts andSite-Specific Base Substitutions

摘要

The authors used colony probe hybridization and PCR/DNA sequence analysis todetermine the mutations in approximately 1,640 revertants of the -1 frameshift allele hisD3052 and approximately 260 revertants of the base substitution allele hisG46 of Salmonella typhimurium induced by the heterocyclic amine cooked food mutagen 2-amino-6-methyldipyrido(1,2-a:3',2'-d)imidazole (Glu-P-1). All of the mutations were at sites containing guanine, which is the base at which Glu-P-1 forms DNA adducts. A hotspot mutation involving the deletion of a CG or GC within the sequence CGCGCGCG accounted for 100% of the Glu-P-1-induced mutations at the frameshift allele in strains TA1978 (uvr(+)) and TA1538 (delta uvrB) and 99% in TA98 (delta uvrB, pKM101). To explain the induction of these hotspot mutations by Glu-P-1, the authors describe here a more detailed version of their recently proposed correct incorporation/slippage model. (Copyright (c) 1994 Wiley-Liss, Inc.)