Differentiation and Development in Plant Cell Cultures

摘要

Differentiation in plant cell cultures was studied by tissue culture and geneticapproaches. The influence of various environmental factors on differentiation was elucidated using Digitalis lanata protoplasts in the first part of the study. The molecular genetics of differentiation were studied in embryogentic birch (Betula pendula Roth.) cell cultures in the second part. Isolation and culture methods were developed for Digitalis lanata seedling protoplasts. The physiological state and the age of explant proved to be critical for protoplast yield and viability. Suitable osmotic pressure, high protoplast density in the beginning of culture, gentle enzyme treatment and efficient purification procedures were essential for protoplast regeneration. In addition, the use of agarose as a gelling agent of the medium was an essential factor for the initiation and maintenance of cell division.