Highly Expressed 'Trichoderma reesei' Genes: Cloning, Characterization and Use inProtein Production on Glucose-Containing Media

摘要

The filamentous fungus Trichoderma reesei is probably the best studiedcellulolytic organism and is widely used by the biotechnical industry for production of cellulolytic and xylanolytic enzymes. Accumulating data on the production of mammalian and heterologous fungal proteins by T. reesei suggest that it is also applicable for production of heterologous proteins. In this work, T. reesei was further developed as a versatile host organism by using the techniques for molecular biology. The work is divided into two parts. A method was first developed which allows the isolation of genes that are strongly expressed in the desired culture conditions, and the promoters of two of the isolated genes were used to construct an expression system for glucose-based cultures in T. reesei. The second part dealt with the biochemical and genetic characterization of hydrophobins in T. reesei.