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Rapid Targeting and Measurement of Microorganism Digestive Activity

机译:快速靶向和微生物消化活性的测量

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Fluorogenic substrates incorporating micron-diameter polymeric particles havebeen synthesized and investigated as a novel means of quantitating enzyme activity in the digestive tract of microorganisms. This project involved obtaining initial experimental evidence that this new technology (enzyme probes) can reduce, and in some cases eliminate, the limitations of using soluble enzyme substrates for toxicity measurements in whole organisms. Brachionus calyciflorous (rotifer) and Nannochloris (green algae) were used in these studies. It was clearly demonstrated that the enzyme probes based upon micron-diameter particles could be ingested by the rotifers, facilitating simultaneous measurement of ingestion rate and enzyme activity.

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