Bioaugmentation with 'Burkholderia cepacia' PR1 301 for In situ Bioremediation ofTrichloroethylene Contaminated Groundwater

摘要

A pilot field study was conducted at the Moffett Federal Airfield, Mountain View,CA, to determine whether effective in situ aerobic cometabolic biodegradation of trichloroethylene (TCE) could be accomplished through bioaugmentation with a genetically modified strain of Burkholderia cepacia G4 (G4) together with feeding of lactate to serve as an energy and growth substrate for the organism. Strain G4 is highly effective at TCE cometabolism but requires either phenol or toluene to induce oxygenase enzyme activity. A strain of G4 was developed through NTG mutagenesis that constitutively expresses the oxygenase so that no inducer need be added. The modified strain, B. cepacia PR1(sub 301) (PR1 (sub 301)), can degrade TCE effectively while growing on simple substrates such as lactate. Strain-specific molecular probes were developed for monitoring the presence and movement of PR1 (sub 301) and were based upon rep-PCR analysis.