The funding provided by the National Institute of Justice was used to develop a cellular sorting method (antibody/magnetic bead reagent capture system) for the recovery of sperm from sexual assault evidence. The design of the antibody/magnetic bead capture system was such that: (1) It utilized differentiating features (extracellular antigenic sites unique to sperm) so that the separation of cell types would be complete; (2) It would provide a more efficient means of sperm recovery, increasing the yield of DNA from the sperm fraction; and (3) The DNA isolation process would be compatible with the amplification of the CODIS core STR loci. The project was funded for one year and was given a no cost extension to December 31, 2001. The long term goal would be to develop an efficient, reliable and cost effective system for the automation of the recovery of sperm from sexual assault evidence.
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