Extraction, Cleanup, and Gas Chromatography/Mass Spectrometry Analysis of Sediments and Tissues for Organic Contaminants

摘要

This document describes the analytical methods developed and used by the Environmental Conservation Division of the National Marine Fisheries Services Northwest Fisheries Science Center for the analysis of sediment and tissue samples for nanograms per gram concentrations of selected aromatic hydrocarbons and chlorinated hydrocarbons. Detailed descriptions are presented for sample extraction, cleanup by silica/alumina columns and sizeexclusion high-performance liquid chromatography, and analysis by gas chromatography/mass spectrometry, as well as the calculation of analyte concentrations and important quality assurance measurements. Detailed descriptions are also given for determining the percent dry weight of tissues and sediments, and for determining the percent total extractable content of tissues. Information pertaining to the necessary laboratory supplies and instruments is provided. In addition, to facilitate use of this document by other laboratories, the step-wise procedures are presented in a descriptive manner.