Rapid and Sensitive Determination of Bacteria in Water Using Nanoparticles

摘要

A series of new silica-based fluorescent nanoparticles were developed in this work. These nanoparticles can simultaneously emit fluorescence signals at different wavelengths. The principle of fluorescence resonance energy transfer (FRET) has been employed in making the fluorescent nanoparticles. The conditions for synthesis of the nanoparticles were optimized. These fluorescent nanoparticles have shown excellent photostability due to the protection of the silica nanomatrix. The surface of the multicolored fluorescent silica nanoparticles were functionalized using antibodies for recognizing pathogenic bacterial cells. Based on the antigen and antibody reaction, the nanoparticles were linked to the specific bacterial cells. Therefore, different types of bacterial cells were differentiated by the corresponding antibody-functionalized fluorescent silica nanoparticles.