The purpose of this verification is to evaluate the performance of a wastewater rapid toxicity technology that could be used to monitor industrial or domestic wastewater. The verification protocol covers two products from the same vendor, both are acute toxicity tests with luminescent bacteria. In the bioluminescence testing with LUMIStox and ECLOX, a strain of naturally occurring luminescent bacteria, Vibrio fischeri, is used. Vibrio fischeri is a non-pathogenic, marine, luminescent bacterium which is sensitive to a wide range of toxicants. When properly grown, luminescent bacteria produce light as a by-product of its cellular respiration. Any inhibition of cellular activity results in a decreased rate of respiration and a corresponding decrease in the rate of luminescence. The light emission/luminescence can be measured with a LUMIStox or ECLOX luminometer. Inhibition of the light emission in the presence of a sample is determined against a non-toxic control. Verification parameters will include determination of the criterion of detection, the range of application, precision as measured by repeatability and reproducibility, agreement with accepted values, and robustness. Other parameters such as an evaluation of the user manual, product costs, and environmental and occupational health and safety aspects will also be included.
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