Development of the In-Vitro Lymphocyte-Lymphocyte Interaction as a Test for Histocompatibility

摘要

Studies of a tissue matching system are being done using the cell-culture interaction of live lymphocytes from prospective tissue recipients and a sonicated cell extract from prospective donors. The present phase emphasizes methodology in the areas of: (1) Cell isolation to give high yield, controlled composition and high livability. (2) Evaluation of optimal culture conditions, including the source of serum, and the use of chemically defined media with insulin and hydrocortisone supplementation. (3) The development of a uni-directional reaction using live cells from a prospective graft recipient and a sonicated antigenic cell lysate from a prospective donor. (4) The evaluation of present techniques used for assessing the reaction and the development of new, more direct techniques. (5) Rabbit and hamster lymphocytes are being studied because of the fav orable populations for the study of genetic differences that can be readily obtained in such species. (Author)