Research and Development of Adenovirus Vaccines and Parainfluenza Virus Vaccines and Adaptation of Respiratory Syncytial Virus to Embryonated Eggs of Several Avian Species

摘要

Nine type 3 and nine type 5 experimental adenovirus vaccines were prepared and tested: four from GMK-propagated and five from WI-38-propagated viruses of each type. Comparisons were made between crude, concentrated, and subunit vaccines. Of these 18 vaccines, only two failed potency tests in guinea pigs. In general, WI-38 vaccines were superior to GMK vaccines. Amniotic fluids were collected from embryonated eggs infected with parainfluenza types 1, 2, and 3 for the preparation of a trivalent vaccine. The following lots have been completed: Trivalent parainfluenza vaccine; diphtheria and tetanus toxoids alumprecipitated, pertussis and trivalent parainfluenza virus, types 1, 2, and 3, combined; parainfluenza type 1 virus vaccine skin test antigen. Stability tests on trivalent parainfluenza virus vaccine were accomplished. A uniform rate of decrease in potency was noted for these vaccines when stored at 4C for nine months. Another lot of trivalent parainfluenza virus vaccine is in process. Attempts to adapt the respiratory syncytial virus vaccine to embryonated hen's egg were unsuccessful. (Author)