Studies on Improving and Standardizing the Process for Preparation of Cryoprecipitated Antihemophilic Factor (Human).

摘要

Cryoprecipitate was made from blood which had been stored at 4 C for various intervals of time from 1to 72hours. Ten bags of cryoprecipitate were made for each chosen storage interval. Factor VIII levels were measured on donor plasma frozen within an hour of the donation,on the plasma just after its separation from red cells on each cryoprecipitate after it was thawed for use,on each pool of ten bags of cryoprecipitate,and on the plasma of the recipient of the pool before and after the infusion. Cryoprecipitates prepared from blood stored for 1to 24hours before centrifugation were of nearly the same potency,and that cryoprecipitate prepared from blood stored 48to 72hours had about 60% as much Factor VIII as cryoprecipitate made from fresh blood.