Development of Sensors to Monitor Blood Constituents in vivo.

摘要

The ultimate objective of this program was the preparation and testing of insertable sensors for blood-plasma ions based on lipid bilayer elements. Self-supporting bilayers held in ca. 1sq mm plastic frames,prepared according to the procedures of Mueller and Rudin from sphingomyelin/tocopherol and n-decane/oxidized cholesterol,were investigated. Reinforcing media were sought in which to imbed the bilayers to compensate for their mechanical fragility. However,as with hydrogel supports of agarose and polymerized 2-hydroxyethl-and 2,3-dihydroxypropylmethacrylates investigated in the first year of this program, bilayers broke in the course of polymerizing or forming a polyacrylamide hydrogel support. Replacing the hydrogel with liquid glycerol provided mechanical support,but destroyed the valinomycin-and other antibiotic-sensitized ion-sensing bilayer properties of interest. A study of an alternative preparative approach was begun whereby a lipid monolayer was cast on a sheet of polyacrylamide hydrogel for making a lipid-centered sandwich of two such coated sheets.