Coordinated Studies of Platelet and Protein Reactions on Artificial Materials.

摘要

Studies are reported of the adhesion of blood platelets and the adsorption of the proteins albumin,fibrinogen,and gamma globulin to various surfaces. The surfaces were placed on discs which were spun either in pools of canine blood anticoagulated with heparin or in human plasma or in solutions of human-proteins. Platelet adhesion was determined by counting through the microscope en face;protein adsorption was determined by using either radiolabeled proteins followed by counting of activity on the surface or by post-exposure treatment of surfaces which had adsorbed unlabeled proteins with labeled specific antibodies to these proteins,followed by counting of activity on the surface. The effect of speed and spinning rate on platelet adsorption was studied;the reproducibility of the procedure was established statistically;and the effect of pre-wetting of the surface was studied experimentally and theoretically. Uptake of proteins and platelets varied markedly from surface to surface with an apparent correlation between platelet and fibrinogen uptake.