Artificial Kidneys:Biochemical Evaluations.

摘要

PLasma from uremic patients and normal subjects was grossly fractionated into proteins,free and conjugated amino acids (Peak II),and uric acid. Fractionation was assessed by flow-monitoring at 280nm, immunoelectrophoresis, fluorescamine and phospho-tungstate reactivity,and measured content of amino acids before and after hydrolysis of collected fractions. Conjugated amino acids before and after hydrolysis of collected fractions. Conjugated amino acids of Peak 11were very low in normal subjects compared with uremic patients. The 280-nm absorption was significantly correlated with the total content of conjugated alpha-amino N2,but not with that of the unconjugated alpha-amino N2. Generation rates of the Peak II materials were directly related to the generation rates of urea,but not with those of creatinine. Thus,Peak II materials were derived from catabolism of ingested protein as is urea. Clearances of the Peak II materials by experimental high-flux membranes were not found to exceed those measured for cuprophane. Conjugated amino acids were separated from amino acids of Peak II by ligand exchange chromatography and were further fractionated on Sephadex G-15.