Immobilized-Enzyme,Continuous-Flow Reactor Incorporating Continuous Electrochemical Regeneration of NAD,

摘要

Electrochemical regeneration of the cofactor nicotinamide adenine dinucleotide (NAD) has been coupled with the alcohol-dehydrogenation reaction which consumes NAD and produces NADH using alcohol dehydrogenase bound to alumina. Alcohol (reactant) is added directly to the system while aldehyde (product) leaves the system through an ultrafiltration membrane which prevents loss of the cofactor. This system provides a continuous-flow process for carrying out a cofactor requiring enzymatic reaction with no net loss or consumption of enzyme or cofactor and without the use of reagents for regenerating the cofactor. Although the process shown here is not economically practical,it may be a harbinger of useful and technically feasible chemical reaction systems based on immobilized enzymes requiring cofactors.