Laser Methods of Rapid Detection,Identification,and Quantitation of Human Enteric Viruses in Sewage and Rivers.

摘要

This study has shown that it is possible to obtain Raman spectra of poliovirus in water solution. Since a Raman spectrum can be obtained in minutes or even seconds,in principle,it has been demonstrated that very rapid analysis of viruses is possible. Details of virus preparation,purification,and concentration have been discussed. Polio I spectra are similar to those of plant viruses studied previously,but are sufficiently different to allow spectra to be used for identification purposes. Problems of instrument sensitivity and rapid sample preparation have to be overcome before a virus analysis machine based upon Raman spectroscopy can be used routinely.