Preparation and Evaluation of Immobilized Single and Multiple Enzyme Systems.

摘要

The study demonstrated that the enzymes lactase,papain and lipase could be immobilized,either singly or concomitantly,on derivatized Sepharose 4-B (4% agarose gel beads). Sepharose 4-B was activated by cyanogen-bromide and then allowed to react with ethylenediamine and succinic anhydride to obtain succinylaminoethyl agarose. The enzymes were coupled to the succinylaminoethyl agarose with a water insoluble carbodiimide reaction. The effects of immobilization on the pH and temperature of assay and kinetic parameters such as apparent Michaelis constant and apparent maximal velocity for each enzyme were investigated. It was felt the immobilized multiple enzyme system could be successfully used for the hydrolysis of lactose,proteins and lipids in cheese whey.