A Rapid Method for Estimating Log P for Organic Chemicals,

摘要

A rapid,inexpensive technique based on reverse-phase high pressure liquid chromatography has been developed to estimate the n-octanol/water partition coefficient of organic chemicals. The system consists of a preparative Micro-Pak C-(R)10reverse-phase column eluted with a 15percent water/85percent methanol solvent flowing at 2ml/min at room temperature. The chemicals are detected in the eluant with a standard ultraviolet detector or a fraction collector system followed by appropriate analysis of the fractions to determine the retention time of the chemical. A linear calibration of the logarithm of retention time with the logarithm of the partition coefficient (Log P) is attained by using a mixture of benzene,bromobenzene,biphenyl, bibenzyl,pp'DDE,and 2,4,5,2',5' pentachlorobiphenyl as reference standards of known Log P. Chemicals with Log P of approximately 3elute in less than 10min whereas those with Log P greater than 5elute after 20min.