Current Intelligence Bulletin 24. Direct Black 38, Direct Blue 6, and Direct Brown 95 Benzidine-Derived Dyes

摘要

The National Institute for Occupational Safety and Health (NIOSH) recommends that three widely used benzidine-derived dyes, Direct Black 38, Direct Blue 6, and Direct Brown 95, be handled in the workplace as if they were human carcinogens. This recommendation is based primarily upon a preliminary analysis of National Cancer Institute (NCI) data from short-term feeding studies, and on early results from NIOSH field studies. Cancerous and precancerous liver conditions were found in rats, similar to the damage produced by known liver carcinogens. Degeneration of liver cells was found in mice. Although the dyes tested by NCI contained less than 4 ppm residual benzidine when fed to the test animals, greater quantities of benzidine were found in the urine of dosed rats and mice. Caution is also indicated by preliminary results from NIOSH field studies showing that humans working with these same dyes also excrete higher than expected levels of benzidine in their urine. Both laboratory and field studies indicate that these benzidine-derived dyes can be metabolized to benzidine which is present in the urine of animals and humans. Based on the data from the short-term study, NCI scientists believe a cancer-causing potential exists upon exposure to the benzidine-derived dyes, most likely through the mechanism of metabolic conversion of the dyes to benzidine in the animal system. This NIOSH/NCI Joint Bulletin summarizes the results of the NCI animal study, the NIOSH field studies, other pertinent data, their implication for occupational health, and suggested guidelines for minimizing employee exposure to the three dyes.