Investigation of the Chemical Identity of Soluble Organophosphorus Compounds Found in Natural Waters.

摘要

Four algal species (Chlamydomonas reinhardtii,Chlorella pyrenoidosa, Anacystis nidulans,and Anabaena flos-aquae) were grown in batch culture on 32p labelled media to yield dissolved organic phosphorus (DOP) compounds containing a radioactive tag. The DOP compounds of filtered culture solutions were characterized by Sephadex gel filtration and thin layer chromatography (TLC) as a function of culture age. Additional TLC of individual Sephadex fractions was conducted. Time,culture and known compounds (inositol mono-and hexaphosphate) comparisons were made. High performance liquid chromatography was used to separate inositol mono-and hexaphosphates and to compare the DOP components of one algal species (C. reinhardtii) with inositol phosphates. Combinations of alkaline bromination and Sephadex pretreatment were examined.