Development and Evaluation of a Two-Step Membrance Filter Method for Fecal Coliform Recovery in Chlorinated Sewage Effluents.

摘要

The standard one-step M-FC broth membrane filter (MF) procedure for recovery of fecal coliform (FC) in chlorinated wastewater effluents is much less effective than the multiple-tube (most probable number, MPN) technique. A two-step MF method was developed. The results of 126comparisons using chlorinated effluents from five wastewater plants showed that FC recovery with the two-step MF method is comparable to that with the MPN procedure. In the development of the two-step MF method for FC recovery in chlorinated effluents,only the Millipore HC filters were used. Literature shows that different bacteria recovery capabilities are reported for various membranes. Therefore,nine other types of membranes were compared with Millipore HC for their fecal coliform recovery in chlorinated effluents from the five wastewater treatment plants. The results of 61comparisons were analyzed by Duncan's multiple range test.