Chemical Synthesis and Biological Applications of Nucleic Acids to Nitrogen Fixation.

摘要

The purpose of this research is the development of new and improved methods for the chemical synthesis of oligodeoxyribonucleotides and their application to gene transfer studies involving biological nitrogen fixation. The use of the pivaloyl group for the protection of the amino group of guanine is described and compared to the previously used isobutyryl group. The use of the t-butyldimethylsilyl group in the preparation of N-acylated monomers and as a protecting group for the 3'and 5'hydroxyl function is also examined. These techniques were applied to the scaled up synthesis of protected monomers. A description of the use of the methyl group for the protection of the 3'-5' phosphodiester link is presented,as is a discussion of further investigations of a new chain elongation procedure involving the use of phosphites. The initial studies covering solid phase support and plans to increase overall efficiency by use of chain elongation reactions on a solid phase support are included.