Detection of Enteroviruses and Bacterial Pathogens Surviving Standard Sewage Treatment.

摘要

A commercially available dialysis unit,the Amicon DC-30 Diaflo unit,was evaluated for its utility in concentrating enteric viruses and bacteria from the effluents of a sewage treatment plant. The unit's efficiency for virus recovery was evaluated by seeding tap water samples with enteroviruses and then refining the concentration procedure so that acceptable virus recovery (>25%) was attained. However,many difficulties were encountered when sewage effluents were examined,most notably the poor flow rates and the poor virus recovery. The particulate matter present in the effluent apparently adsorbs much of the virus that is present which is then removed with the suspended solids as the sample passes through the unit. The bacterial quality of the effluents was surprisingly good with coliform counts of some samples meeting recreational water standards,however,Shigella,Salmonella,Klebsiella,and Proteus species were occasionally isolated from the effluents. Major conclusions are (a) the Amicon DC-30unit has only limited application for the concentration of enteroviruses from sewage effluents due to poor virus recovery rates and (b) the occasional isolation of viruses and pathogenic bacteria from the effluents indicates that some pathogens survive secondary sewage treatment.