Comparative Kinetic Studies of Phosphate-Limited Growth and Phosphate Uptake in Phytoplankton in Continuous Culture.

摘要

A comparative kinetic study of phosphate-limited growth and phosphate uptake was carried out in chemostat cultures of Anabaena flos-aquae Lyng. Breb., Ankistrodesmus falcatus (Corda) Ralfs, Asterionella formosa Hass., Fragilaria crotonensis Kitt., and Microcystis sp. Lemm. For each test organism the growth rate (microns) can be described as a function of total cell phosphorus (P), i.e. cell quota (q), or hot-water-extractable P (q sub sp). Phosphate uptake rate in each species examined was a function of external P concentration (S) and intracellular P levels. The substrate parameter for uptake (K sub m) remained constant at all growth rates, but the apparent maximum uptake rate (V sub m) increased with decreasing microns, or q, in all test organisms.