Effect of Methyl-n-Butyl Ketone and Other Keton Homologues on Mammalian Cells in Culture

摘要

The effects of methyl-n-butyl-ketone (591786) (MNB) and other ketone homologues were studied in mammalian cells. Human and rat fibroblast cultures were grown in serum and Eagle's medium. In 1 to 2 week old cultures with unmyelinated neurons and Schwann cells, the neurons were extremely sensitive and the Schwann cells were very resistant to ketone intoxication. The order of toxicity was 2,5-hexanedione (110134), MNB methyl-amyl-ketone (110430) (MA), methyl-ethyl-ketone (78933) (ME) and acetone (67641). The metabolite of MNB was 200 to 300 times more than acetone. The author suggests that the ketones MNB, 2,5-hexanedione and MA may induce chemical and structural alterations in sensitive cells. These compounds have a specific effect on proteins and could modify enzyme functions. The authors conclude that the data necessary to determine the mechanism of ketone action resulting from this study is insufficient. These studies have demonstrated that lines of human neuroblastoma can be used to assay the toxic effects of ketones because of their extreme sensitivity to the ketones.