Initiation/Promotion Assay for Chemical Carcinogens in Rat Liver

摘要

A short-term initiation/promotion bioassay has been developed in rat liver using putative preneoplastic foci as the endpoint for the detection of chemical carcinogens. The two protocols of the bioassay used in this study were varied according to the time 2/3 partial hepatectomy was performed in relation to when the initiator or test substance was given. After 7 weeks of promotion with phenobarbital in the drinking water, the rats were killed and the liver was sectioned, stained, and scored for gammaglutamyl transpepitdase (GGTase)-positive foci.