Mucus Glycoprotein Secretion by Tracheal Explants: Effects of Pollutants

摘要

Tracheal slices incubated with radioactive precursors in tissue culture medium. The authors have used anin vitro approach, a combined method utilizing exposure to pneumotoxins in vivo coupled with quantitation of mucus secretion rates in vitro, to study the effects of inhaled pollutants on mucus biosynthesis by rat airways. In addition, the authors have purified the mucus glycoproteins secreted by rat tracheal explants in order to determine putative structural changes that might be the basis for the observed augmented secretion rates after exposure of rats to H2SO4 aerosols in combination with high ambient levels of ozone.