Detection of Occupational Exposure to Genotoxic Agents with a Urinary Mutagen Assay

摘要

The levels of mutagenic activity in the urine of 198 smoking and non-smoking chemical workers, coke plant workers and controls were measured using a urinary mutagen assay based on the Ames Salmonella/microsome test. Urine samples were concentrated using the non-polar resin XAD-2 eluted with methylene chloride and acetone. Activity towards Salmonella strains TA1538 and TA100 with and without microsomal preparation S-9 was measured. Regardless of occupational exposure, smokers had considerably higher urinary mutagenic activity than non-smokers on Strain TA1538 with S-9, and to a lesser extent on Strain TA100 with S-9.