Look at Techniques for the Dimensional Calibration of Standard Microscopic Particles

摘要

This report surveys generic techniques for the dimensional calibration of microscopic particle size standards. It notes some user needs and available instrumentation, documentary standards, and commercial, sized particles. It discusses the elements of the measurement process, including the particle and medium, size-dependent phenomena, shape factors, single-number indices of average size, and means of length calibration. The report examines systematic differences among published results of electron microscopy, light scattering, particle counter, and other measurements on widely-used commercial polymer spheres. It concludes that the definitive calibration of narrow-distribution polymer spheres, a prerequisite first step toward calibration of irregularly-shaped matter, would best involve a three-element approach involving the techniques named.